Semi-Quantitative Analysis of HOXA11, Leukemia Inhibitory Factor and Basic Transcriptional Element Binding Protein 1 mRNA Expression in the Mid-Secretory Endometrium of patients with endometriosis

نویسندگان

  • Marzieh Farimani Sanoee
  • Massoud Saidijam
  • Naser Shokrzadeh
چکیده مقاله:

Background: HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation. We also describe a semi-quantitative RT-PCR protocol optimized in our laboratory. Methods: Eight patients with endometriosis were considered as our case and 8 fertile women as control group. Expression levels of HOXA11, LIF and BTEB1 mRNA were measured in endometrium during the mid-secretory phase using semi-quantitative RT-PCR. Results: We describe the detailed procedure for the analysis of HOXA11, LIF and BTEB1 mRNA levels. Endometrial HOXA11 and LIF mRNA expression levels (normalized to ß-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls at the time of implantation (P<0.05). A similar trend was seen in BTEB1 mRNA expression. Conclusion: The results suggest that the alteration in expression pattern of the some genes could account for some aspects of infertility in endometriosis.

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منابع مشابه

semi-quantitative analysis of hoxa11, leukemia inhibitory factor and basic transcriptional element binding protein 1 mrna expression in the mid-secretory endometrium of patients with endometriosis

background: hoxa11 and leukemia inhibitory factor (lif) and basic transcriptional element binding protein1 (bteb1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. in this case-control study, the expression pattern of these mrna was evaluated in patients with endometriosis at the time of implantatio...

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Semi-quantitative analysis of HOXA11, leukemia inhibitory factor and basic transcriptional element binding protein 1 mRNA expression in the mid-secretory endometrium of patients with endometriosis.

BACKGROUND HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation...

متن کامل

Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility

BACKGROUND A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile ...

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عنوان ژورنال

دوره 15  شماره 3

صفحات  66- 72

تاریخ انتشار 2011-11

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